Abstract: In this paper, Talaromyces sp. DYM25 isolated from the Yap Trench in the Western Pacific Ocean was used as the experimental strain. The extracellular product of strain DYM25 was extracted by water-saturated n-butanol, and an alkaloid chromogenic reaction was used to examine the components of the crude extracts of the n-butanol phase. The antimicrobial, antioxidant and antitumor activities of the extracellular product were also investigated. The results showed that one liter of strain DYM25 fermentation broth could yield approximately 2 g of extract after extraction and evaporation with water-saturated n-butanol. The alkaloid chromogenic reaction showed that the alkaloid salts presented in the extracellular product. The growth of Fusarium equiseti which was a pathogenic fungus of Fusarium wilt on cucumber could be inhibited by the extracellular product of Talaromyces sp. DYM25. This study is the first report of Talaromyces about antagonistic fungus of F. equiseti. Meanwhile, it is found that the extracellular products also possessed certain antioxidant with antitumor activities. Its antioxidant capacity could be enhanced with the increasing product concentration. The inhibitory ability against human pancreatic cancer cell PANC-1 was also concentration dependent. The diverse biological activities of Talaromyces sp. DYM25, as result, highlight its value in the research and in application prospect.