Abstract: In this study, DNA barcoding technique was used to describe the species diversity and distribution of oysters from the coast of Zhejiang Province, China. A total of 550 oyster samples were collected from 7 locations along the Zhejiang coastal area, from which 232 samples were randomly selected for genomic DNA extraction and barcoding analysis. Based on the mitochondrial DNA endcodedcytochrome c oxidase I (COI) gene and nuclear 28S ribosomal RNA (28S rRNA) gene, 232 specimens were identified as 10 species of 3 genera. In the genus Crassostrea, 5 species were identified as C. sikamea (127 samples), C. angulata(64 samples), C. nippona (4 samples), C. ariakensis (1 sample) and C. gigas(1 sample). In the genus Ostrea, 2 species were identified as O. circumpicta (4 samples) and O. denselamellosa (2 samples). In the genus Saccostrea, 3 species were identified as S. kegaki(20 samples), S. echinata(8 samples) and S. sp.(1 sample). The results indicated that there is a high diversity of oyster species along the coast of Zhejiang, where the diversity differs in both latitudes and the distance from land. C.sikamea and C.angulata are dominant species in the coast of Zhejiang.