Abstract: A new family B DNA polymerase （ppa_PolB） from thermophilic archaea strain Palaeococcus pacificus DY20341 was recombinantly expressed and purified. The dUTPase （ppa_dUTPase） from the strain was also obtained. Using a serial of ppa_PolB and ppa_dUTPase combinations， we tested their performance in amplifications of fragments from archaea， bacteria and eukaryote. One of the combinations successfully amplified the Palaeococcus pacificus DY2034 six agarase genes， which could not be achieved by commercial DNA polymerase. We concluded the necessary of expanding DNA polymerases for variety of goals in PCR due to this new DNA polymerase combination．