Abstract: Base medium of Pseudomonas was screened for producting pyoverdines among four kinds of media， it was showed that sodium succinate medium as basic medium and the effects of additions of different components on the output of pyoverdines were examined. The results showed that the production of pyoverdines could be promoted significantly， while glycerin， sea salt and NH₄NO₃ added in sodium succinate medium. Sodium succinate comound medium was screened and optimized by the orthogonal experiment: 1.5% glycerol， 4 g/dm³sodium succinate， 6 g/dm³ K₂HPO₄， 3 g/dm³ KH₂PO₄， 1 g/dm³ （NH₄）₂SO₄， 1 g/dm³ NH₄NO₃， 5 g/dm³ sea salt. The highest production of pyoverdines is 265 mg/dm³ and more than 7 times than before. The medium is simple and advantageous to isolate and purify the pyoverdines.At the same time， the fermentation conditions were optimized and the best conditions were determined. The results showed that initial pH， inoculation amount， temperature， shaking speed and the time were 6.9，1.0%，25℃，220 r/min and 28h， respectively．