Abstract: Interleukin 17 receptor （IL-17R） family play important roles in IL-17 cytokine family mediated immune responses. IL-17RE-like is a novel IL-17 receptor whose ligand has not been found yet to date. In this paper， we cloned an IL-17REL homologue from large yellow croaker and named it as LycIL-17REL. The deduced open reading frame （ORF） of LycIL-17REL is 1 659 nucleotides （nt）， encoding a protein of 552 amino acids （aa） which consists of a signal peptide， an extracellular domain， a transmembrane region and an intracellular domain. The extracellular domain of LycIL-17REL possesses a FN I-like domain， while intracellular domain loses the SEFIR domain which is conserved in IL-17R family. Amino acid homology comparison shows that LycIL-17REL has 57-0% identity to Fugu（Takifugu rubripes） IL-17REL. Based on the structural and phylogenetic analysis， the vertebrate IL-17R family members were suggested to be classified into two groups: the IL-17RA/RB/RD group and the IL-17RC/RE/REL group. Besides this， we speculated that vertebrate IL-17REL and IL-17RE were probably differentiated the same ancestor from species older than teleosts. LycIL-17REL was constitutively expressed in all tissues tested， especially in mucosal related tissues such as intestine and gills. The up-regulated expression of LycIL-17REL was observed in head kidney and gills of large yellow croaker stimulated by Aeromonas hydrophila. These results suggested that LycIL-17REL may play an important role in the immune response against bacterial infection.