Abstract: Deep-sea fungi are an important componentof deep-ea micmorganisms and playvital roles in deep-seae-cosystems.However,the knowledgeabout fungi in deep-sea envionments is limited.In this study,15 samples indeepsea sedimentsfromthreeocean areas(South Atlantic Ocean,East Pacifc Ocean,and Southwest Indian 0-cean)were collected forthediversity study by culture-dependent approach.A total of175 fungal strains were isola-ted,including93 yeast strains and 82 filamentous fungi strains.Thephylogenetic analysisofIIS sequenceof fungalisolates showed that the yeasts belong to 2 species,Rhodosporidium paludigenum and Rhodotorula mucilaginosa.Eighty-two filamentous fungal strains were affiliated with 15 fungal genera,includingAspergillus,Cladosporium,Pemicilliam,Aternaria,Fusarium,Acremonium,Phoma,Tritirachium,Chaetomium,Erophiala,Engyodontium,Sistotema,Schizophyllum,Tlletiopsis and Hormonema.Aspergillus accounting for 47.6%ofthetotal filamentousfungal isolatesisa dominant genus.The resultsrevealed that a high diversity of fungi in deep-sea sediments oftheoceansand haveprovided a keyinsight into the community composition of fumgi in deep-sea environments