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基于转录组测序技术的海月水母足囊生殖分子机制研究
徐鹏臻,李勇学,孙婷婷,彭赛君,董志军
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(中国科学院烟台海岸带研究所牟平海岸带环境综合试验站,山东 烟台 264003;中国科学院大学,北京 100049)
摘要:
足囊生殖是海月水母 (Aurelia coerulea)抵御恶劣环境的一种无性生殖方式,对维持和扩增海月水母种群具有重要作用,目前对海月水母足囊产生的分子机制尚不清楚。本研究首次构建了海月水母产囊和非产囊螅状体转录组的表达谱,并结合生物信息学比较分析,探究驱动足囊生殖的分子机制。转录组测序共检测到44 873个基因,筛选过滤后共发现200个差异表达基因,其中95个基因在产囊螅状体中显著上调,105个显著下调。本研究挖掘出了卵黄蛋白原(基因VTG-1和VTG-2)和角蛋白(基因Hornerin)等调控海月水母足囊营养储备及几丁质形成的潜在功能基因。此外,GO和KEGG分析结果显示ErbB信号通路、Hedgehog信号通路等参与细胞分裂和分化的生物学过程在产囊螅状体中显著下调,基底膜组织、运动正调节等参与细胞迁移的的生物学过程在产囊螅状体中显著富集,表明这些生物学过程在足囊形成过程中发挥着重要作用。综上,本研究为海月水母螅状体在恶劣环境下的适应机制研究提供了理论依据。
关键词:  海洋生物学  海月水母  螅状体  足囊生殖  休眠  转录组
DOI:10.3969/J.ISSN.2095-4972.20230116002
基金项目:国家科技基础资源调查专项(2022FY100603);国家自然科学基金委山东联合基金重点支持项目(U2106208);泰山学者青年专家项目(tsqn202211263)
Molecular mechanism of podocysts reproduction in the moon jellyfishAurelia coerulea based on transcriptome sequencing
XU Pengzhen,LI Yongxue,SUN Tingting,PENG Saijun,DONG Zhijun
(Muping Coastal Environment Research Station, CAS, Yantai 264003, China;University of Chinese Academy of Sciences, Beijing 100049, China)
Abstract:
As an asexual reproduction mode of moon jellyfish Aurelia coerulea podocysts play an important role in resist hostiling environment, maintaining and expanding the population of polyps. However, relevant genes that drive podocysts forming are still unclear. This study developed the transcriptome profile and performed bioinformatics analyses between podocysts forming and non-forming polyps for the first time, and explored molecular mechanism of podocysts formation. A total of 44 873 genes were detected by transcriptome sequencing, while 200 differential genes were harvested after filtration, of which 95 genes were significantly up-regulated and 105 genes were significantly down-regulated in podocysts forming polyps. Further analysis discovered vitellin genes (VTG-1 and VTG-2) and hornerin genes (Hornerin) may regulate the nutrients reserve and chitin formation of podocysts. In addition, GO and KEGG analysis showed that biological processes involved in cell division and differentiation such as ErbB pathway and Hedgehog pathway were significantly down-regulated in podocysts forming polyps, while process that involved in cell migration such as basement membrane organization, positive regulation of locomotion were significantly enriched, indicating the processes played an important role in podocysts formation. Thus, this study provides a theoretical basis for the research of adaptive mechanisms when Aurelia coerulea polyps facing with hostile environment.
Key words:  marine biology  Aurelia coerulea  polyps  podocysts  diapause  transcriptomics

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