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两种海藻寡糖促乳酸菌增长的转录组比较研究
陈兴麟,李丽,林河通
0
(1.自然资源部第三海洋研究所,福建 厦门 361005; 2. 福建农林大学食品科学学院,福建 福州 350002)
摘要:
本研究采用全自动生长曲线分析仪Bioscreen C,比较了不同浓度的新琼四糖、新琼六糖、龙须菜发酵液、低聚果糖、低聚木糖、葡萄糖对乳酸菌Lactobacillus plantarum的促增长作用。结果显示,葡萄糖和龙须菜发酵液最佳的添加浓度分别为0.4 g/L和0.8 g/L,而新琼四糖、新琼六糖、低聚果糖、低聚木糖均为0.6 g/L。在各自的最佳浓度下,促生长效果最好的依次为新琼四糖、低聚果糖、新琼六糖、龙须菜发酵液、低聚木糖和葡萄糖。进一步比较在最佳添加量(0.6 g/L)和低浓度(0.2 g/L)条件下,新琼四糖和新琼六糖对乳酸菌Lactobacillus plantarum的转录组的影响,发现新琼四糖和新琼六糖在促乳酸菌生长的作用机理上有很大的相似性,当浓度升高时对细胞内的“转运活性”有更高的影响。
关键词:  海洋生物学  新琼四糖  新琼六糖  乳酸菌  差异表达基因  GO注释
DOI:
基金项目:国家海洋局第三海洋研究所基本科研业务费资助项目(2017025)
Comparative study of transcriptomes from Lactobacillus promoted by two algal oligosaccharides
CHEN Xing-lin,LI Li,LIN He-tong
(1. Third Institute of Oceanography, MNR, Xiamen 361005, China; 2. Fujian Agriculture and Forestry University, College of Food Science, Fuzhou 350002, China)
Abstract:
In this study, automatic growth curve analyzer Bioscreen C was used to compare the effects on growth promoting of Lactobacillus plantarum by different concentrations of neoagarotetraose, neoagarohexaose, fermentation broth of Gracilaria lemaneiformis, fructo-oligosaccharide, xylo-oligosaccharide and glucose. The results showed that the optimal concentrations of glucose and fermentation broth of Gracilaria lemaneiformis were 0.4 g/L and 0.8 g/L, respectively, while those of neo-agarose, neo-agarose, fructo-oligosaccharide and xylo-oligosaccharide were all 0.6 g/L. Compared in each optimum concentration, the promoting effect on Lactobacillus plantarum growth ranked from high to low was neoagarotetraose, fructooligosaccharide, neoagarohexaose, fermentation broth of Gracilaria lemaneiformis, xylo-oligosaccharide and glucose. Compared the transcriptome of Lactobacillus plantarum under the conditions of optimum addition (0.6 g/L) and low concentration (0.2 g/L) of neoagarotetraose and neoagarohexaose, the differentially expressed genes were found similar to GO annotation results, which had higher influence on “transporter activity”when the oligosaccharide concentration increased.
Key words:  marine biology  neoagarotetraose  neoagarohexaose  Lactobacillus  differentially expressed genes  gene ontology annotation

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