| 摘要: |
| 利用单因素实验对海洋解木糖赖氨酸芽孢杆菌（Lysinibacillus xylanilyticus sp. JZ008）培养条件进行了优化.优化后的培养条件：温度为35℃、pH值为7.5、接种量为3%（V/V）、装液量为50 cm3/250 cm3时，菌量达到2.01×108 CFU/cm3，比优化之前提高了40.6%.添加海藻寡糖可以促进菌株生长，添加量为5.0 g/dm3时发酵液中活菌数是对照组的2.51倍.研究了菌株JZ008对Cd2+、Cr3+、Cu2+ 3种重金属离子的吸附特性，结果表明菌株JZ008对3种重金属离子污染的水溶液吸附率效果明显，20 d吸附率分别达到95.6%、96.4%、87.0%.土壤重金属吸附实验结果表明，海藻寡糖复配菌株JZ008组对Cd2+、Cr3+、Cu2+ 吸附效果明显，60 d吸附率分别达到82.5%、82.0%、86.9%.菌株JZ008对重金属的优良吸附作用为进一步开发解木糖赖氨酸芽孢杆菌在重金属修复方面的应用奠定了基础. |
| 关键词: 海洋生物学 解木糖赖氨酸芽孢杆菌 优化 海藻寡糖 重金属 吸附 |
| DOI：10.3969/J.ISSN.2095-4972.2018.03.011 |
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| 基金项目:国家海洋局第三海洋研究所基本科研业务费专项资金资助项目（海三科2016029）；海洋公益性行业科研专项资助项 |
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| Biosorption of heavy metals cadmium, chromium, copper by marine bacterium Lysinibacillus xylanilyticus strain JZ008 |
| LIN Fan-yu,WANG Run-ping,YI Zhi-wei,LEI Kai-yu,CHAN Zhu-hua |
| (Third Institute of Oceanography, SOA, Xiamen 361005, China;School of Marine Sciences, Ningbo University, Ningbo 315211, China;BGI Clinical Laboratories (Shenzhen) Co., Ltd，Shenzhen 518083, China) |
| Abstract: |
| The fermentation conditions of marine bacterium Lysinibacillus xylanilyticus strain JZ008 were optimized with single factor methodology. The optimal fermentation conditions were set at temperature 35℃, pH 7.5, inoculation quantity 3.0% (V/V) and volume of liquid 50 cm3/250 cm3. After optimization, the amount of bacterium reached 2.01×108 CFU/cm3, which was 40.6% higher than that before optimization. Algae oligosaccharide is helpful for the growth of bacterium. The viable count of fermenlatin broth added 5.0g/dm3 algae oligosaccharide reached 2.51 times comparing to the control group. The biosorption characteristics of Cd2+, Cr3+, Cu2+ by strain JZ008 were studied. The results showed that the biosorption rate of three heavy metals was obvious in solution, the adsorption rate reached 95.6%, 96.4% and 87.0% after 20 days, respectively. The results also showed that the biosorption rate of the heavy metals was obvious in soils mixed with algae oligosaccharide and strain JZ008. The adsorption rate reached 82.5%, 82.0% and 86.9% after 60 days, respectively. The excellent biosorption characteristics of the strain laid a foundation for the further development and application of Lysinibacillus xylanilyticus in the remediation of heavy metals. |
| Key words: marine biology Lysinibacillus xylanilyticus optimization algae oligosaccharides heavy metal biosorption |
