| 摘要: |
| 为研究DNA条形码技术在蛇鳗科鱼类分类鉴定中的可行性,选取了蛇鳗科7属10种36个个体进行COⅠ基因扩增,结果共获取36条基因序列,平均长度为655bp,序列中T､C､G､A碱基的平均含量分别为:27.50%､28.10%､26.00%､18.40%,A+T含量均高于50%.样品种内､种间和属间的遗传距离(K2P)分别为:0.52%､20.07%､23.96%,遗传距离随着分类阶元的提高而增大,种间遗传距离是种内遗传距离的38.59倍,符合Hebert提出的相差10倍的物种鉴定标准,表明COⅠ基因序列可以作为蛇鳗科鱼类分类鉴定的有效DNA条形码. |
| 关键词: 海洋生物学 蛇鳗科 细胞色素C氧化酶亚基Ⅰ基因 DNA条形码 物种鉴定 |
| DOI：10.3969/J.ISSN.2095-4972.2017.03.015 |
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| 基金项目:国家海洋局第三海洋研究所基本科研经费资助项目(海三科2013001);海洋公益性行业科研专项经费资助项目(200705025,201005020-2);厦门市海洋与渔业局资助项目(12PZP001SF10-5) |
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| Suitability analysis of mitochondrial COⅠ gene used as DNA barcode for Ophichthyidae |
| ZHANG Zhi-lan,LIN Ru-rong,XING Bing-peng |
| (Third Institute of Oceanography, SOA, Xiamen 361005, China) |
| Abstract: |
| In this study, we collected DNA barcodes based on COⅠ sequences from 36 individuals of 10 species in 7 genera of Ophichthyidae. The mean length of the sequences was 655 bp and the average content of T, C, G and A was 27.50%, 28.10%, 26.00% and 18.40%, respectively. The A+T content was higher than 50% in every species. The average Kimura two parameter (K2P) genetic distances of interspecies, intraspecies and genus were 0.52%, 20.07% and 23.96%, respectively. K2P genetic distance values were found increasing with taxonomic level. Interspecific genetic distance was about 38.59 times higher than the differences of intraspecies, which met Hebert's species identification standard (interspecies genetic distance was more than 10 times of intraspecies). As high efficiency of species identification is demonstrated in the present study by DNA barcoding, we conclude that COⅠ sequencing can be used to identify fish species. |
| Key words: marine biology Ophichthyidae COⅠ DNA barcoding species identification |
