摘要: |
荔枝螺是福建海域常见食用螺类,古方记载和民间长期食用证实具有良好药用价值,但国内外对其生物活性物质的研究还较少.本研究以福建海域疣荔枝螺(Thaisclavigera)为研究材料,从疣荔枝螺消化腺部位获得抗癌组分EEA2,通过液相色谱、气质联用色谱分析发现其主要成分为6溴靛红(6bromoisatine),分子结构式C8H4BrNO2.EEA2能有效抑制人乳腺癌MCF7、人肝癌QGY7703细胞增殖,药物浓度高于40μg/cm3时,癌细胞存活率显著降低.EEA2能诱导肝癌细胞QGY7703发生PARP蛋白剪切,加入泛caspase抑制剂使细胞存活率显著提高.同时,EEA2使MCF7细胞核产生固缩,线粒体膜电位显著下降,凋亡细胞数量显著增加.EEA2对肝癌细胞QGY7703的IC50为46μg/cm3,对乳腺癌细胞MCF7的IC50为28μg/cm3,对癌细胞的增殖抑制作用主要是以促凋亡方式实现的. |
关键词: 海洋生物学 疣荔枝螺 抗肿瘤活性 溴靛红 细胞凋亡 |
DOI: |
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基金项目:国家自然科学基金青年项目资助项目(41306167);海洋公益性行业科研专项资助项目(201205024) |
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Anti-tumor activities in vitro of rock shells, Thais clavigera |
ZHANG Fang,YANG Shanjun,WANG Zhaokai,YANG Longhe,WU Peng,HU Fan,YU Xianwen,LIN Xiangzhi |
(Engineering Research Centre of Marine Biologial Resource Comprehensive Utilizatian.Third Institute of Oceanography,?SOA.Xiamen 361005.China;Fujian Agiriculature and Forestry University. College of Food Science, Fuzhou 350002. China) |
Abstract: |
Rock shells, Thais clavigera, as one of the most popular seafood in Fujian Province, are proved to have pharmaceutical activities according to the traditional Chinese medicine records and the historical usage. However,
there are few public reports about the active components from the snail. In this paper, we isolated and screened the anti-tumor substances E-EA-2 from the rock shells. The main component of E-EA-2 was identified as 6-bromoisatine by means of HPLC and GC-MS. The cancer cell proliferations ( MCF-7 and QGY-7703 ) were significantly inhibited when the concentration of E-EIA-2 exceeded 40 g/cm3. E-EA-2 could induce PARP cleavage in QGY-
7703 cells. While the number of survival cancer cells obviously increased at presence of cell permeable pan caspase inhibitor Z-VAD. And cultured MCF-7 cells with E-EA-2 after 24h, cell nuclear shrinkage, loss of mitochondrial membrane potential and cell apoptosis were obvious. The IC50 of E-EA-2 was 46 ug/cm3 and 28g/cm3 to QGY-7703 and MCF-7, respectively. The anti-tumor eflects of E-EA-2 might be taken through promoting cell apoptosis. |
Key words: marine biology Thais clavigera anti-tumor activity 6-bromoisatine cell apoptosis |