| 摘要: |
| 利用人工培育的2龄四指马鲅（Eleutheronema tetradactylum）亲鱼自然产卵受精而获得的受精卵在实验室中孵化和培育，对受精卵胚胎发育到仔鱼孵出的全过程进行详细的观察记录.结果表明：四指马鲅受精卵呈圆球形，卵膜光滑，单油球.平均卵径675.78 μm，油球平均直径258.46μm.在水温为31～33℃，盐度为27.50～28.00，pH值为8.2条件下，胚胎发育用时13 h 52 min，可分为卵裂、囊胚、原肠胚、胚体形成、器官分化和孵化6个阶段，初孵仔鱼全长1.46±0.25 mm，3DAH仔鱼全长2.42±0.18 mm，口和肛门张开，仔鱼开口摄食. |
| 关键词: 海洋生物学 四指马鲅 受精卵 胚胎发育 |
| DOI：10.3969/J.ISSN.2095-4972.2016.03.012 |
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| 基金项目:广东省科技计划资助项目（2016A030303029，2014A020217010）；广东省海洋渔业科技推广专项科技攻关与研发资助项目（A201401A05）；中央级公益性科研院所（中国水产科学研究院南海水产研究所）基本科研业务费专项资金资助项目（2014YD04） |
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| Observation on embryonic development of Eleutheronema tetradactylum |
| XIE Mujiao,OU Youjun,WEN Jiufu,LI Jiaer,ZHOU Hui,WANG Pengfei,LIU Qiqi |
| (Key Laboratory for Exploitation & Utilization of Marine Fisheries Resource in South China Sea， Miniatry of Agriculture; South China Sea Fisheries Research Institute， Chinese Academy of Fishery Sciences， Guangzhou 510300， China;College of Fisheries and Life Sciences， Shanghai Ocean University， Shanghai 201306， China) |
| Abstract: |
| The embryonic development of Eleutheronema tetradactylum is studied in this paper. Zygotes fertilized naturally were obtained from 2 years old cultured E. tetradactylum during the processing of natural spawning. The result shows that mature zygote of E.tetradactylum is round with an oil ball in the center， the average diameter of zygote is 675.78μm， and the oil ball average diameter was 258.46μm. The embryonic development finished in 13 h 52 min under 31~33℃ at salinity 27.50～28.00 and pH 8.2. The process of embryonic development is fast which can be divided into 6 stages， including cell division stage， blastula stage， gastrula stage， embryo development stage， organs differentiation stage and hatching stage. Body length of newly hatched larvae is 1.46±0.25mm， while 3DAH larvae is 2.42±0.18mm with the mouth and anal opening， being able to feeding initiatively. |
| Key words: marine biology Eleutheronema tetradactylum fertilized zygote embryonic development |
