| 摘要: |
| 实验培养条件下，分析比较了干露后喷施植物生长调节剂吲哚乙酸（IAA）和萘乙酸（NAA）对野生型坛紫菜（Pyropia haitanensis）叶状体生长的影响.IAA处理结果显示：与对照组相比，20、10 mg/dm3 IAA处理都明显促进坛紫菜叶状体生长（p<005），且20 mg/dm3处理效果优于10 mg/dm3，而100 mg/dm3处理组抑制生长明显（p<005）.培养25 d 时，20 mg/dm3 IAA处理组叶状体平均长度增加了354%，叶绿素a、藻红蛋白和藻蓝蛋白含量分别增加了122%、399%、291%.NAA处理结果显示：15、10 mg/dm3 NAA处理组对坛紫菜叶状体生长都有明显促进作用（p<005），且15 mg/dm3处理效果优于10 mg/dm3，而40、80 mg/dm3处理组出现抑制效应，其中80 mg/dm3处理组抑制生长较明显（p<005）.培养25 d 时，15 mg/dm3处理组叶状体平均长度增加了352%.与对照组相比，虽然低含量组（05、10、15、20 mg/dm3）的叶绿素a含量无显著差异（p>005），但是藻红蛋白和藻蓝蛋白含量均明显增加，以15 mg/dm3处理组含量最高，分别增加了426%和439%.而高浓度处理组（40、80 mg/dm3）叶绿素a含量显著降低（p<005），且80 mg/dm3处理组的藻红蛋白和藻蓝蛋白含量明显低于对照组（p<005）.因此，连续干露条件下喷施适宜浓度的IAA（20 mg/dm3）和NAA（15 mg/dm3）均可有效地促进野生型坛紫菜叶状体的生长． |
| 关键词: 海洋生物学；坛紫菜 叶状体 植物生长调节剂 喷施 生长 |
| DOI：10.3969/J.ISSN.2095-4972.2016.03.010 |
|
| 基金项目:福建省种业创新与产业化工程资助项目(2014S1477 10); 福建省自然科学基金资助项目(2014J01095);福建省海洋高新产业发展专项资助项目(闽海洋高新2014 19);福建省属公益类科研院所基本科研专项资助项目(闽海渔科2013R001 1); 福建省海洋与渔业结构调整专项资助项目;福建省海洋经济创新发展区域示范资助项目(2014FJPT01);厦门南方海洋研究中心资助项目(14PZY017NF17，15GZY021NF04);浙江省科技计划资助项目(2016F50033) |
|
| Effects of spraying two plant growth regulators on the growth of blades under the condition of desiccation in Pyropia haitanensis |
| ZHONG Chenhui,LIN Qi,SONG Wulin,LIU Yanfei,ZHANG Peng,LIN Xiangyang,HUANG Ruifang |
| (Fujian Fisheries Research Institute and Fujian Key Laboratory of Cultivation and Highvalue Utilization of Marine Organisms， Xiamen 361013， China;Fujian Marine Economic Operation Monitoring and Assessment Center， Fuzhou 350003， China;Zhejiang Mariculture Research Institute， Wenzhou 325005，China) |
| Abstract: |
| In experimental cultures， the effects of intervally spraying 2 plant growth regulators （IAA and NAA） on the blade growth of Pyropia haitanensis during desiccation were compared. The results showed that， compared to the controlled group， both 20 and 10 mg/dm3 IAA group had significant effect promoting the growth of blades （p<005）， and the blades treated with 20 mg/dm3 group had better growth than those of 10 mg/dm3group， while the treatment of 100 mg/dm3 IAA inhibited the growth of blades significantly （p<005）. After 25 days of culture， the mean length of blades treated with 20 mg/dm3 IAA increased by 354%， and the contents of chlorophyll a， phycoerythrin and phycocyanin increased by 122%， 399% and 291%， respectively. Furthermore， compared to the controlled group， both 15 and 10 mg/dm3 NAA treatments promoted the growth of blades significantly（p<005）， and the blades in 15 mg/dm3 group grew better than those of 10 mg/dm3 group， but the 40 and 80 mg/dm3 NAA group presented significant inhibition effect（p<005）. After 25 days of culture， the mean length of blades treated with 15 mg/dm3 NAA increased by 352%. Compared to the controlled group， lower concentration of 05，10，15 and 20 mg/dm3 had no significant difference in chlorophyll a contents， but had higher contents of phycoerythrin and phycocyanin （p>005）. Especially， 15 mg/dm3 NAA group had higher contents of phycoerythrin and phycocyanin， which increased by 426% and 439%， than those of the controlled group， respectively. However， the contents of chlorophyll a， phycoerythrin and phycocyanin in 40 and 80 mg/dm3 NAA group were much lower than those of the controlled， particularly in 80 mg/dm3 NAA group （p<005）. The results indicated that spraying optimum concentration of IAA （20 mg/dm3） and NAA （15 mg/dm3） at the interval of desiccation would be benefit for promoting the blade growth of P. haitanensis． |
| Key words: marine biology Pyropia haitanensis blades plant growth regulator spraying growth |
