Abstract: The tidal creek network is a vital component of material transport, hydrological regulation, and ecological functioning in mangrove wetlands, and its spatiotemporal evolution plays a key role in ecosystem stability and management. Based on Landsat imagery from 1986 to 2024 of the Dongzhaigang Mangrove Nature Reserve in Hainan, this study employed visual interpretation to analyze the spatial distribution and temporal evolution of the tidal creek network. Results show that primary creeks dominate the overall network configuration, and their expansion or contraction determines the system’s structural evolution. The evolutionary process of tidal creeks can be divided into a degradation stage (1986–1995), a low-fluctuation stage (1995–2013), and a recovery stage (2013–2024). The stability of the creek system exhibits a positive correlation with regional area and a negative correlation with aquaculture disturbance. At the same time, its response to human activities varies with the combined effects of aquaculture disturbance intensity and regional area. Air temperature and offshore wave dynamics are identified as the main natural drivers of creek development, whereas vegetation expansion inhibits creek complexity. Although precipitation is not a statistically significant effect, its environmental dependence may exert dual effects. Overall, the evolution of the Dongzhaigang tidal creek system results from the coupled effects of natural dynamics and human interventions. Its spatial heterogeneity reflects the interactive regulation between ecological restoration and environmental conditions, providing a scientific basis for the conservation of tidal creeks and the management of mangrove wetlands.