Abstract: To mitigate the adverse impacts of high temperatures and rot diseases during the initial cultivation stage of Neoporphyra haitanensis in autumn, an inter-seasonal refrigerated net technology was employed in this experiment. Specifically, refrigerated nets were revived and cultured in the sea during the following year based on climatic and marine conditions, thereby alleviating the negative effects of high temperatures and rot diseases on cultivation while enhancing the utilization efficiency of sea areas and increasing production cycles. Distinct from conventional short-term refrigerated net techniques, this study tested inter-seasonal cultivation after prolonged refrigeration (180 d) and analyzed the nutritional composition of first-harvested N. haitanensis. Results showed that the cultivation cycle for first-harvested N. haitanensis using inter-seasonal refrigerated nets was 25 d, shorter than the 35 d using autumn seedling nets. Although the yield of the inter-seasonal group was slightly higher than that of the autumn seedling group, the difference was not statistically significant (P＞0.05). Similarly, significant differences (P＜0.05) were observed in phycocyanin content, protein, fat, and carbohydrates. These findings confirm the feasibility of inter-seasonal refrigerated net cultivation for the N. haitanensis, with no notable decline in overall quality. This study provides a foundation for promoting the application of inter-seasonal refrigerated net technology in N. haitanensis cultivation.