Abstract: This study was based on a seasonal macrobenthos surveys conducted in nearshore waters of northern Jiangsu from 2021 to 2022. By combining environmental factors and comparing them with historical data, the community structure and spatiotemporal distribution of macrobenthos were analyzed, as well as their correlation with environmental factors. Results showed that 241 species of macrobenthos were identified in four seasons with dominant annelids (91 species), arthropods (72 species), and mollusks (47 species). The dominant species mainly were Paralacydonia paradoxa, Mactra iridescens, Protankyra bidentata, Alpheus japonicus, and Glycinde gurjanovae. The annual average density was (381±147) ind/m², and the average density in spring and winter was higher than that in summer and autumn. The annual average biomass was (54.98±21.75) g/m², and the average biomass in spring was slightly higher than the other three seasons. Correlation analysis showed that though the main combinations of environmental factors affecting the community structure of macrobenthos varied slightly in 4 seasons, the overall water depth, water temperature, dissolved oxygen, some water quality parameters (salinity, nitrate and nitrite content, etc.), and sediment type (particle size, organic carbon content) might still be the main factors affecting community composition and distribution. Comparative analysis with surrounding sea areas show that the community characteristics of this sea area were similar to some neighboring sea areas. Comparative analysis shows that the biomass of macrobenthos in this sea area was lower than that of neighboring Jiaozhou Bay and Rushan Bay, and the community shows a certain trend of miniaturization. It suggests that it may relate to environmental disturbances such as marine engineering construction, aquaculture, and input of terrestrial pollutants in the surrounding areas. It is necessary to strengthen environmental monitoring, reduce interference appropriately, and take appropriate protection measures.