Abstract: The concept of standardization guideline for constructing a marine ecological monitoring station present in this paper is to accelerate the process of networking and standardization of long-term, fixed-point and continuous monitoring of marine ecosystem besides designation. Based on the analysis of status and experience of various long-term ecological monitoring networks home and abroad, the research status and existing problems of long-term positional monitoring of marine ecosystems in China were discussed, as well as the significance, principles and objectives of constructing marine ecological monitoring stations. In addition, guidelines of the principles, processes, construction contents and operational maintenance and management of the marine ecological monitoring stations were given. It is of great theoretical and practical significance in promoting the healthy and orderly development of marine ecological monitoring and its network system, enhancing the monitoring and early warning ability and research quality of marine ecosystems.