Abstract: In order to improve the breeding efficiency of seahorse, Hippocampus abdominalis, and understand the effects of temperature, pairing mode, stocking density and breeding density on the growth, survival and reproduction, we studied the effects of different temperatures and different sex ratios on the seedling yield, and the effects of different breeding densities on the growth and survival of the seedlings. The results showed that the suitable breeding water temperature for broodstock was 16－19 ℃. The best ratio of male to female fish ranged from 1∶1 to 2∶3, resulting in relatively high seedling yield. In the early stage (within 30 days) the optimal density was 0.5－1.0 ind./L, whereas in the middle stage (between 30 and 60 days) it was 0.25－0.50 ind./L. Our study can be taken as scientific reference for artificial propagation and seedling in H. abdominalis rearing.