Abstract: The acute and chronic salinity stresses on the survival and non-specific immuno-enzymetic activity of large yellow croaker (Larimichthys crocea) were investigated. In the acute experiment, the superoxide dismutase (SOD) activity in the liver of croaker peaked in 1 day (p<0.05) and dropped in 3 and 7 days, and the catalase (CAT) activity showed a decrease-increase-decrease trend under low salinity stress. The acid phosphatase (ACP) activity in the serum of croaker dropped significantly in 1 day (p<0.05) and increased gradually in 3 days and recovered in 7 days, while the alkaline phosphatase (ALP) activity increased in 1 day, 3 days and normalized in 7 days. The lysozyme (LZM) content in the serum of croaker fluctuated and it peaked at the first day and dropped later. In the chronic experiment, no significant differences for SOD, CAT, ALP, ACP and LZM were observed compared with the control group. The results suggested that the croaker be more tolerant to chronic low salinity stress, while acute salinity dropping significantly affected the non-specific immunity of the fish, thus drastic changes in salinity should be avoided in farming.