Abstract: Taken laminarin extraction rate as an index, the technological conditions of laminarin extraction from laminaria processing wastes were optimized by twostage method of microwave and compound enzyme. When taking laminaria raw material 2.00 g, the optimal technological conditions for the microwave stage were as follow: the microwave extraction solution was pH 6.5, the ratio of liquid to substrate was 60.00 mL/g with 12 min 400 W microwave treating at 60 ℃. Under these optimal conditions, the extraction rate of laminarin could reach 106.49 g/kg. The optimal technological conditions for the compound enzyme stage were as follow: the compound enzyme extraction solution was pH 6.00, the ratio of liquid to substrate was 40.00 mL/g and the enzyme treated for 105 min at 55 ℃ with the dosage of combined enzymes including cellulase 4.00×10³U, papain 4.00×10³U and pectinase 2.40×10³U. Under these optimal conditions, the extraction rate of laminarin could reach 115.16 g/kg. Under the optimal conditions of the microwave and compound enzyme assisted extraction, the extraction rate of laminarin could reach (208.93±0.18) g/kg. The results of antioxidant activity showed that the extracted laminarin had certain antioxidant capacity besides a stronger scavenging activity of hydroxyl (·OH).