Abstract: Phenol is a great threat to marine ecological system, so it is of great significance to isolate phenol-degrading bacteria characterizing higher effectiveness and higher adaptation of marine environment. In present work, phenol-degrading bacteria were enriched and isolated from rhizosphere soil of mangrove around Beibu Gulf. A total of 30 strains were isolated and purified. As a result, five strains named FGYS1, FGYS7, FGYS11, FGYB1 and FGYB5 were obtained. It shows that they could tolerate 70 g/L high concentration of sodium chloride and degrade phenol up to 1 500 mg/L. They were identified by morphological observation, BOX-PCR diversity analysis,16S rRNA and gyrB gene sequence analysis. All of them were Pseudomonas. FGYS1, FGYS7 and FGYS11 are consistent in 16S rRNA gene sequence. The nucleotide sequence similarity of 16S rRNA gene to those of Pseudomonas extremaustralis 14-3^T and Pseudomonas meridianaCMS 38^T in the EzBioCloud data base was 99.71% and 99.64%, respectively. It is the same for the strains FGYB1 and FGYB5, the similarity of 16S rRNA gene to those of Pseudomonas veroniiDSM 11331^T and Pseudomonas extremaustralis14-3^T was both 99.64%, respectively. The phylogenetic tree analysis of 16S rRNA gene showed that FGYS1, FGYS7, FGYS11, FGYB1 and FGYB5 located in the same branch with P. veronii, but relatively far away from other model strains of Pseudomonas. The suitable growth conditions for phenol degradation by strain FGYS1 were 25-35 ℃, pH 4-6 and the sodium chloride concentration less than 60 g/L. While for strain FGYB1, the conditions for phenol degradation were 25-35 ℃, pH 4-6, and the sodium chloride concentration less than 100 g/L. FGYS1 and FGYB1 could also degrade catechol, hydroquinone and resorcinol simultaneously. These phenol-degrading bacteria exhibited potential utilization in the disposition of aromatic pollution and the restoration of environment in mangrove ecosystem.