Abstract: Lysozyme is an important molecule in the innate immune system and it participates in many immune responses. In the lysis process,a hydrolysis system is formed to destroy and eliminate the pathogens to promote the immune defense of the body. In this study,the gene EgLyz of i-type lysozyme was first cloned from the Amphipod Eurythenes gryllus,which lives in the hadal zone. Sequence analysis revealed that EgLyz had low homology with the known sequence and contained a destabilase domain. The result of comprehensive analysis indicates that EgLyz gene may encode a novel lysozyme protein. Furthermore,the EgLyz was cloned and expressed in prokaryotic E. coli. Thus,a soluble recombinant EgLyz with high purity was obtained. The study will establish a basis for further study on properties and functions of the EgLyz.