Abstract: Using the specific-locus amplified fragment sequencing (SLAF-seq) technology, we developed 47 high polymorphic microsatellite markers for Acanthopagrus latus, among which 17 were di-nucleotide repeats and the other 30 were tri- to hexa-nucleotide repeats. The allele number per locus ranged from 2 to 27 (mean 10), and the observed and expected heterozygosities varied from 0.156 to 0.938 (mean 0.682) and 0.177 to 0.963 (mean 0.741), respectively. The polymorphism information contents (PIC) ranged from 0.166 to 0.946 (mean 0.705). After Bonferroni correction, 43 microsatellite loci were consistent with Hardy-Weinberg equilibrium (HWE), while the other 4 loci deviated from the HWE. These polymorphic microsatellite markers could provide new and effective molecular markers for the genetic resources study of A. latus. The results of cross species amplification showed that a total of 31 microsatellite markers could be successfully amplified in 9 species of family Sparidae. Among them 17 were transferable in Acanthopagrus pacificus, Acanthopagrus schlegelii and Acanthopagrus australis, and they would provide new markers for the future studies of phylogenetic relationship and population genetics of these species. The other 3 markers were also transferable in Evynnis cardinalis, Pagrus major, Pagrus caeruleostictus and Dentex hypselosomus.