Abstract: In this study we used molecular biology methods to clone the gene of IHHNV capsid protein and construct prokaryotic expression vector. We screened the polypeptide with high antigen characteristics to immunize rabbits for preparing efficient and specific IHHNV polyclonal antibodies. Finally, we established a new highly efficient double antibody sandwich ELISA method to detect shrimp IHHNV. The results showed that the antigen polypeptide of the IHHNV capsid protein gene of shrimp virus was successfully expressed and highly sensitive polyclonal antibodies were prepared. When used the antigenic peptide and polyclonal antibodies for the detection of ELISA, the titer of ELISA was more than 1∶12 800, and the lowest antigen protein of 1pg could be detected. Our research showed that the highly efficient double antibody sandwich ELISA method has been successfully established, which can have important value for the shrimp culture and the prevention and control of IHHNV virus disease.