| 摘要: |
| 本研究采用硅胶柱层析、高效液相色谱等方法对深海真菌Aspergillus puniceus A2发酵产物进行了化学成分分离纯化,通过核磁共振等现代波谱学方法,同时结合相关文献进行数据分析,鉴定化合物的结构。从A. puniceus A2中分离鉴定出6个呫吨酮并双呋喃环类化合物,化学结构分别确定为Austocystin I（1）、Austocystin G（2）、6-methoxyl Austocystin A（3）、Austocystin F（4）、F02ZA-1593B2（5）、Austocystin A（6）,化合物1~6均为首次从该真菌中分离得到。通过细胞毒性和抗炎活性评价发现化合物1、2、4和6在20 μmol/L浓度时,对肝星状细胞LX2显示出毒性作用,提示其可能有抗肝纤维化的作用；化合物2、3、5和6在20 μmol/L浓度时,能显著抑制LPS诱导小鼠单核巨噬细胞RAW264.7细胞分泌NO,显示出一定的抗炎活性。 |
| 关键词: 海洋生物学 深海真菌 Aspergillus puniceus 呫吨酮并双呋喃环类化合物 细胞毒活性 抗炎活性 |
| DOI：10.3969/J.ISSN.2095-4972.2023.02.003 |
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| 基金项目:福建省科技计划农业引导性（重点）项目（2020N0030）；国家自然科学基金（82073763） |
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| Secondary metabolites of a deep-sea-derived fungus Aspergillus puniceus A2 and their potential in drug development |
| CAI Songyan,HE Jianlin,NIU Siwen,HONG Bihong |
| (College of Food Sciences ?? Technology, Shanghai Ocean University, Shanghai 201306, China;Technology Innovation Center for Exploitation of Marine Biological Resources, Third Institute of Oceanography, MNR, Xiamen 361005, China) |
| Abstract: |
| The fermentation product of a deep-sea-derived fungus Aspergillus puniceus A2 was isolated and purified with silica gel column and highperformance liquid chromatography, which isolated 6 compounds. The chemical structures of the 6 compounds were identified as Austocystin I(1), Austocystin G(2), 6-methoxyl Austocystin A(3), Austocystin F(4), F02ZA-1593B2(5), and Austocystin A(6) by NMR spectrum analysis and compared with literature data. All the isolated compounds were reported firstly in this fungus.Among them, compounds 1, 2, 4 and 6 showed toxic effects on hepatic stellate cells LX2 at the concentration of 20 μmol/L, suggesting that they may have anti-hepatic fibrosis effects.Compounds 2, 3, 5 and 6 inhibited significantly the LPS-induced NO secretion in mouse monocyte macrophage RAW264.7 at 20 μmol/L, showing anti-inflammatory activity. |
| Key words: marine biology deep-sea fungus Aspergillus puniceus xanthone-bisfuran rings cytotoxic activity anti-inflammatory activity |
