| 摘要: |
| C型凝集素作为凝集素家族的主要成员之一，在无脊椎动物中有着丰富的多样性，其独特的生物学功能广泛应用于生物、医药等诸多领域。本研究从采集于东北太平洋深海热液区胡安·德富卡洋脊的管状蠕虫Ridgeia piscesae中克隆获得了一种新型C型凝集素基因RPCL。该基因与已报道的凝集素的相似性都很低，并且三维结构预测有异于传统凝集素，提示其可能具有一些特殊的功能性质。进一步的原核表达成功实现了 RPCL的异源重组表达，通过透析复性获得了大量可溶性蛋白，为其相关功能性质的研究打下了坚实的基础。 |
| 关键词: 海洋生物学 凝集素 管状蠕虫Ridgeia piscesae 克隆 表达 |
| DOI：10.3969/J.ISSN.2095-4972.2023.02.002 |
|
| 基金项目:国家重点研发计划（2018YFC0310702） |
|
| Cloning and expression of a novel C-type lectin from tube worm Ridgeia piscesae |
| CHEN Yanjie,RUAN Lingwei,SHI Hong,CHEN Xinhua |
| (Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, China;Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, MNR, Xiamen 361005, China) |
| Abstract: |
| C-type lectin is the main member of the lectin family, which has abundant diversity in invertebrates. Their unique biological functions are widely applied in many fields, such as biology, medicine and so on. In this study, a novel C-type lectin RPCL was cloned from Ridgeia piscesae, a tube worm collected from the deep-sea hydrothermal region at Juan de Fuca Ridge in the northeast Pacific Ocean. The similarity between this gene and the reported lectins is very low, and the predicted 3D structure was quite different from the known lectins, suggesting that it may have some special properties. Furthermore, the heterologous recombinant expression of MRPCL was successfully achieved, and a large amount of soluble proteins were obtained through purification and renaturation. It will facilitate the further study on the function of RPCL. |
| Key words: marine biology lectin tube worm Ridgeia piscesae cloning expression |
