| 摘要: |
| 溶解态无机氮（dissolved inorganic nitrogen， DIN）主要由亚硝酸盐-氮（NO-2-N）、硝酸盐-氮（NO-3-N）和铵氮（NH+4-N）组成，它们在海洋的生物地球化学循环过程中起重要作用。但人类活动向海洋输入了大量无机氮，导致一系列环境问题。为了更好地开展海洋氮循环研究和环境污染管理，需对海水中的DIN进行测定。在众多分析方法中，光谱法因其通用性好、适用范围广、所需设备简单，成为测定海水DIN的首选。本文总结了近10年来基于光谱法测定海水DIN的研究进展，包括紫外分光光度法测定NO-3-N、萘乙二胺分光光度法测定NO-2-N和NO-3-N、次溴酸盐氧化-分光光度法测定NH+4-N、靛酚蓝分光光度法测定NH+4-N、酸碱指示剂-分光光度法测定NH+4-N、荧光法和化学发光法测定DIN等，比较了各分析方法的特点，并展望了光谱法测定海水DIN的发展趋势。总的来说，在分析方法上，新试剂的使用以及一些新合成材料的出现，丰富了DIN的分析手段；在分析仪器上，以流动分析技术为基础的分析仪器在DIN的实验室及现场分析中得到了广泛应用。DIN的分析方法均朝着简单便捷、全自动化、分析速度快、精确度高、可适用范围广的方向发展。 |
| 关键词: 海洋化学 溶解态无机氮 海水 光谱法 测定方法 |
| DOI：10.3969/J.ISSN.2095-4972.2022.04.019 |
|
| 基金项目:国家自然科学基金资助项目（42006166）；国家重点研发计划资助项目（2019YFD0901102）；福建省海洋经济发展专项资金资助项目（FJHJF-L-2021-11）；中国博士后科学基金资助项目（2020M671957） |
|
| Advance in the study of dissolved inorganic nitrogen determination in seawater using spectroscopic methods |
| LIN Kunning,WANG Lianghui,ZHANG Yuanbiao |
| (Key Laboratory of Global Change and Marine Atmospheric Chemistry, TIO, MNR, Xiamen 361005, China) |
| Abstract: |
| Dissolved inorganic nitrogen (DIN) is mainly composed of nitrite (NO-2-N), nitrate (NO-3-N) and ammonium (NH+4-N), which plays an important role in the marine biogeochemical cycle. However, human activities have introduced large amount of inorganic nitrogen and caused many serious environmental problems. In order to study marine nitrogen cycle and manage environmental pollution, it is necessary to determine DIN in seawater. Among many analytical methods, spectroscopic method becomes the top choice for seawater DIN analysis because of good versatility, wide applicability and simple equipment. This review summarizes the research progress in the determination of DIN in seawater based on spectroscopic methods in recent ten years, including UV spectrophotometry for NO-3-N determination, N-1-naphthylethylenediamine dihydrochloride spectrophotometry for NO-2-N and NO-3-N determination, hypobromite oxidation and spectrophotometry for NH+4-N determination, indophenol blue spectrophotometry for NH+4-N determination, acid-base indicator and spectrophotometry for NH+4-N determination, fluorescence and chemiluminescence for DIN determination. The characteristics of each analytical method are compared, and the developmental trend of seawater DIN analysis with spectroscopic method is prospected. In general, for analytical methods, the use of new reagents and the emergence of some new synthetic materials have enriched the analytical means of DIN. For analytical instrument, the instrument based on flow analysis technology is widely used in the laboratory and field analysis of DIN. The analysis methods of DIN are developing in the direction of simple and convenient, full automation, rapid analysis, high accuracy and wide range of application. |
| Key words: marine chemistry dissolved inorganic nitrogen seawater spectroscopy determination method |
