| 摘要: |
| 以大黄鱼（Larimichthys crocea）为研究对象，研究急性、慢性低盐度胁迫对大黄鱼存活状况及非特异性免疫酶活力的影响。结果表明：急性低盐（15、8）胁迫下，在7 d的实验周期中，大黄鱼肝脏的超氧化物歧化酶（superoxide dismutase, SOD）活力呈先上升后下降的趋势，在第1天显著上升（p<0.05）后逐渐下降至显著低于对照组水平；肝脏的过氧化氢酶（catalase, CAT）活力整体呈先下降后上升再下降的趋势，在第1天显著下降后开始升高，第3天后开始下降，至第7天时CAT活力仍显著低于对照组水平；血清中的酸性磷酸酶（acid phosphatase, ACP）活力呈下降后逐渐升高的趋势，在第1 天时显著下降后逐步升高，至第7天时仍显著低于对照组；而血清中的碱性磷酸酶（alkaline phosphatase, ALP）活力在实验开始的第1天到第3天逐渐升高，且均显著高于对照组，至第7天时开始下降至对照组水平；血清中的溶菌酶（lysozyme, LZM）含量呈波动变化，整体呈先上升后下降趋势。慢性低盐（8）养殖14 d后，大黄鱼的各项非特异性免疫酶活力均与对照组无显著性差异（p>0.05）。此外，实验周期内所有组别大黄鱼均未出现死亡，仅急性低盐胁迫组大黄鱼的活动和摄食受到有限影响。大黄鱼对慢性降盐度养殖有较高的耐受能力，而盐度骤降会显著影响大黄鱼的非特异性免疫，实际生产中应避免养殖环境盐度的剧烈变化。 |
| 关键词: 海洋生物学 大黄鱼 低盐胁迫 抗氧化酶 磷酸酶 溶菌酶 |
| DOI：10.3969/J.ISSN.2095-4972.2022.02.020 |
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| 基金项目:福建省科技厅自然科学基金资助项目（2018J05062，2020J02011）；福建省教育厅中青年教师教育科研资助项目（JAT170658）；宁德市科技计划资助项目（20170046，20190009）；宁德师范学院科研经费资助项目（2017Y03） |
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| Effect of low salinity stress on non-specific immunity enzymetic activity of Larimichthys crocea |
| HE Liangyin,SHI Xiaoli,ZHOU Fengfang,CHEN Meixia,HAN Kunhuang |
| (Ningde Normal University, Ningde 352100, China) |
| Abstract: |
| The acute and chronic salinity stresses on the survival and non-specific immuno-enzymetic activity of large yellow croaker (Larimichthys crocea) were investigated. In the acute experiment, the superoxide dismutase (SOD) activity in the liver of croaker peaked in 1 day (p<0.05) and dropped in 3 and 7 days, and the catalase (CAT) activity showed a decrease-increase-decrease trend under low salinity stress. The acid phosphatase (ACP) activity in the serum of croaker dropped significantly in 1 day (p<0.05) and increased gradually in 3 days and recovered in 7 days, while the alkaline phosphatase (ALP) activity increased in 1 day, 3 days and normalized in 7 days. The lysozyme (LZM) content in the serum of croaker fluctuated and it peaked at the first day and dropped later. In the chronic experiment, no significant differences for SOD, CAT, ALP, ACP and LZM were observed compared with the control group. The results suggested that the croaker be more tolerant to chronic low salinity stress, while acute salinity dropping significantly affected the non-specific immunity of the fish, thus drastic changes in salinity should be avoided in farming. |
| Key words: marine biology Larimichthys crocea low salinity stress antioxidant enzyme phosphatase lysozyme |
