| 摘要: |
| 采用单细胞凝胶电泳实验研究了不同浓度的Cu2+(10、50、100、500、1 000、2 500、5 000 μg/L)和不同粒径(0.1、0.2、0.3、0.5、0.7、1.0、2.0 μm)、不同浓度(1、10、15、20、25 mg/mL)的聚苯乙烯微塑料对人单核细胞白血病（Human Acute Monocytic Leukemia, THP-1）细胞的DNA损伤效应，结果表明:单一污染时，Cu2+(≥50 μg/L)对THP1细胞的DNA损伤极显著(p＜0.01)。而且，DNA损伤值随Cu2+浓度的增大而增加。2.0 μm粒径的聚苯乙烯微塑料(10 mg/mL)对THP-1细胞产生明显的DNA损伤效应。复合污染作用下对THP-1细胞的DNA损伤效应，相比单一污染明显增强，而且DNA损伤值比单一污染的理论加和值高。本研究表明Cu2+和聚苯乙烯微塑料均对DNA产生损伤效应，显示一定的基因毒性，二者复合对损伤具有交互增强的作用。 |
| 关键词: 海洋环境科学 DNA损伤 单细胞凝胶电泳 Cu2+ 微塑料 聚苯乙烯 |
| DOI：10.3969/J.ISSN.2095-4972.2022.01.011 |
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| 基金项目:厦门海洋研究开发院资助项目(2018010)；自然资源部第三海洋研究所基本科研业务费资助项目 (HE01-190702(6)） |
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| Studying on DNA damage effects of single and combined contamination of copper ion and polystyrene microplastics on THP-1 cells |
| YIN Yan,WANG Haiyan,HAN Daxiong,LIN Cai,LIN Xihuang,SHAN Bo |
| (Third Institute of Oceanography, MNR, Xiamen 361005, China;School of Pharmaceutical Sciences, Xiamen University, Xiamen 361102, China) |
| Abstract: |
| The effects of different concentrations of Cu2+(10, 50, 100, 500, 1 000, 2 500, 5 000 μg/L) and particle sizes (0.1, 0.2, 0.3, 0.5, 0.7, 1.0, 2.0 μm) of polystyrene microplastics on DNA damage in human acute monocytic leukemia (THP-1 cells) were investigated by singlecell gel electrophoresis experiments. As results, ≥50 μg/L Cu2+ caused extremely significant DNA damage to THP-1 cells (p< 0.01). Moreover, the extent of DNA damage in THP-1 cells increased with increasing Cu2+ concentration. In the other hand, 2.0-μm particle size polystyrene microplastics (10 mg/mL) produced significant DNA damage in THP-1 cells. Under combined contamination, 25 mg/mL microplastics (2.0 μm) and Cu2+(500 μg/L) produced significant DNA damages in THP-1 cells, and the actual effects on DNA damage were much worse than the theoretical combination effects of single contamination. This study indicated that either Cu2+ and polystyrene microplastics caused damage effect on DNA, showing a certain amount of genotoxicity, and the combination of the two had interactive enhancement damage effect on THP-1 cells. |
| Key words: marine environmental science DNA damage comet assay copper ion microplastic polystyrene |
