| 摘要: |
| 为探究藻类形态功能群(Morphologically Based Functional Group， MBFG)在浅水刺参（Apostichopus japonicus）养殖池塘表层底质中的应用以及主要微藻类群密度变化规律，将形态功能群归类方法与冗余分析(Redundancy Analysis， RDA)相结合对刺参养殖池塘两种类型表层底质（泥沙底质和淤泥底质）中的微藻类群形态功能群构成以及主要功能群密度与水温及营养盐的相关性进行分析。结果表明，两种底质中微藻类群仅归为两个形态功能群，分别为形态功能群Ⅴ和Ⅵ，其中形态功能群Ⅵ即硅藻类各月密度较大且与形态功能群Ⅴ差异极显著（p<0.01）；泥沙底质和淤泥底质中硅藻密度分别在9月\[（700 ±17）cells/cm3）\]和8月\[（580±12）cells/cm3]达到峰值， 两池塘上层底质（0～2 cm）中硅藻的密度分别为下层底质（3～5 cm）的3倍（泥沙底质）、25倍（淤泥底质）；冗余分析结果显示，活性硅酸盐浓度与水温分别是影响泥沙底质和淤泥底质中藻类形态功能群Ⅵ密度的首要因素。研究表明，刺参养殖池塘表层底质中微藻种类较少；在浅水刺参养殖池塘表层底质中可应用形态功能群归类方法对微藻类群进行聚类，结合冗余分析研究主要功能群密度的变化规律。养殖时应根据池塘底质类型差异制定相应管理和养护方法。 |
| 关键词: 海洋生物学 形态功能群 硅藻 底质 藻类密度 水温 营养盐 刺参养殖池塘 |
| DOI：10.3969/J.ISSN.2095-4972.2020.03.004 |
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| 基金项目:国家自然科学基金资助项目（31572622，31001113）；山东省重点实验室专项建设计划开放课题资助项目（SDKL2017009-03） |
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| Analysis of algal morphological functional group and their density changes in sea cucumber aquaculture pond |
| SHI Kai,WANG Min,XING Rong-lian,CAO Xue-bin,JIANG Ai-li |
| (College of Life Sciences, Yantai University, Yantai 264005, China;National Alage and Sea Cucumber Project Technology Research Centre, Shandong Oriental Ocean SciTech Co., Ltd., Yantai 264003, China) |
| Abstract: |
| To study the algal morphologically based functional group (MBFG) in surface sediments and to understand the density variation of main microalgae population in the ecosystem of sea cucumber shallow water aquaculture pond, the MBFG classification method combined with the redundancy analysis (RDA) was applied to analyse the distribution of microalgae MBFG on the sandy and muddy surface sediments and the relationships between the densities of main functional groups and factors such as water temperature and nutrients. Results showed that the microalgae populations in two different sediments could be classified into two MBFGs,i.e. group Ⅴ and group Ⅵ, which indicated that the microalgae diversity in the sediments was simple. There was only one diatom specie in functional group Ⅵ accordingly. The monthly diatom density in group Ⅵ, which was much higher, was significantly different from that of functional group Ⅴ (p< 0.01). The diatom densities in the sandy and muddy sediments reached peaks of 700 ± 17 cells/cm3 in September and 580 ± 12 cells/cm3 in August, respectively. The diatom densities in the upper 0-2 cm sandy sediments and the muddy sediments were about 3 times and 25 times higher than that in the lower 3-5 cm sediments, respectively. RDA showed that the primary factor influencing the cell density of the MBFG Ⅵ in sandy sediments was active silicate content, while it was water temperature in muddy sediments. The results showed that MBFG classification method could be applied to cluster the microalgae population functional groups in the surface sediments of sea cucumber aquaculture pond and to analyses the density changes of important functional groups when combined with RDA. Therefore, the corresponding management method and maintenance process should be formulated according to the types of sediments in sea cucumber aquaculture ponds during breeding. |
| Key words: marine biology morphologically based functional group diatom sediments algal density water temperature nutrient sea cucumber aquaculture pond |
