| 摘要: |
| 本研究利用青蛤（Cyclina sinensis）的转录组信息开发微卫星标记，共获得12 418个微卫星位点。其中单核苷酸重复次数最多，共8 422个，占比67.08%；其次是三核苷酸重复，共1 501个，占比12.09%；双核苷酸的重复有1 419个，占比11.43%。在所有重复单元类型中，A/T重复出现频率最高，占单核苷酸重复的48.75%。随机选取81对引物进行微卫星标记验证，最终获得27个具有多态性的微卫星标记。对30个青蛤进行多态性位点检测和评价，共扩增到等位基因99个，每个位点的等位基因数Na为2～6个，期望杂合度He为0.033 3～0.808 5，观测杂合度Ho为0.000 0～0.678 6，多态信息含量（PIC）为0.032 3～0.764 5，有19个位点显著偏离Hardy-Weinberg平衡（p<0.01）。本研究开发的微卫星标记为青蛤的分子遗传学研究、种质鉴定和自然种群资源保护等研究提供了基础数据。 |
| 关键词: 海洋生物学 青蛤 微卫星标记 转录组 |
| DOI：10.3969/J.ISSN.2095-4972.2020.02.008 |
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| 基金项目:国家水产种质资源共享服务平台运行资助项目（2017DKA30470）；温州市种子种苗科技创新专项资助项目（Z20160015） |
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| Development and verification of SSR markers in Cyclina sinensis |
| FANG Jun,SHEN Yan-peng,ZHANG Lei-lei,LI Teng-teng,SHAO Yan-qing |
| (Zhejiang Mariculture Research Institute， Zhejiang Key Lab of Exploitation and Preservation of Coastal BioResource， Engineering Research Center for Marine Bivalves, CAFS， Wenzhou 325005， China;Wenzhou Medical University， Wenzhou 325016， China) |
| Abstract: |
| The transcriptome information of clam，Cyclina sinensis, was used to develop microsatellite markers in this study.After sequencing， 12 418 microsatellite loci were obtained， among them 8 422 mononucleotide repetitions accounted for 67.08% of the total， 1 501 trinucleotide repetitions accounted for 12.09%， and 1 419 dinucleotide repetitions accounted for 11.43%. The poly A/T type was the most frequent poly type accounting for 48.75% of the mononucleotide repetitions. Then， 81 pairs of primers selected randomly were designed for microsatellite loci verification and 27 polymorphic loci were obtained as result. Detection and assessment of polymorphism points were conducted in 30 clams and a total of 99 alleles were amplified. The number of alleles Na at each locus was 2 to 6. The expected heterozygosity （He）， the observed heterozygosity （Ho） and the polymorphic information content （PIC） of each lucus ranked from 0.033 3 to 0.808 5， 0.000 0 to 0.678 6 and 0.032 3 to 0.764 5， respectively. Also， 19 loci deviated significantly from HardyWeinberg equilibrium （p<0.01）. The polymophic ESTSSRs developed in present study will be useful for molecular genetics study， quality evaluation and wildlife resource conservation for the clam C. sinensis． |
| Key words: marine biology Cyclina sinensis microsatellite transcriptome |
