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一种共生藻PCR模板DNA的快速制备法
龙寒,禤金彩
0
(广西民族大学海洋与生物技术学院、广西海洋微生物资源产业化工程技术研究中心、广西多糖材料与改性重点实验室,广西 南宁530008)
摘要:
采用Chelex-100树脂法制备了26个物种(分别属于腔肠动物门、海绵动物门和软体动物门)的共生藻以及一株纯培养共生藻的DNA样品。通过PCR扩增叶绿体23S rRNA基因片段获得了26个有效序列,测序比对结果显示获得的序列与扩增目的基因相符。该方法不仅样品消耗量小、操作时间短、设备需求低廉,而且可以避免使用有害化学试剂。这是一种高效、环保的DNA提取方法,可为共生藻分子研究提供技术参考。
关键词:  海洋生物学  Chelex-100  共生藻  DNA提取
DOI:
基金项目:广西高校中青年教师基础能力提升项目资助项目(2017KY0169,2017KY0166);广西科技重大专项资助项目(桂科AA18242026); 广西科技基地和人才专项资助项目(桂科AD18126005);广西民族大学研究生科研创新项目(gxun-chxzs2018061)
A rapid extraction method for symbiodinium PCR template
LONG Han,XUAN Jin-cai
(School of Marine Sciences and Biotechnology, Guangxi University for Nationalities, Guangxi Marine Microbial Resources Industrialization Engineering Technology Research Center, Guangxi Key Laboratory for Polysaccharide Materials and Modifications, Nanning 530008, China)
Abstract:
DNA samples of symbiodiniums from 26 species of Cnidaria, Spongiatia and Mollusca, and 1 species of pure culture symbiodinium were extracted by Chelex100 resin method. Twentysix valid sequences were obtained by PCR of chloroplast 23S rRNA. BLAST showed that these obtained sequences were consistent with target genes. This method has the advantages of small sample consumption, short operation time and low equipment requirements, and the avoidance of using toxic chemical reagents. It is an efficient and environmentally friendly DNA extraction method and will provide a technical reference for the molecular study of symbiodinium.
Key words:  marine biology  Chelex-100  symbiodinium  DNA extract

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