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黄鳍棘鲷微卫星标记开发及其在鲷科鱼类中的跨物种扩增
吴仁协,翟云,肖瑶,牛素芳,张浩冉,黎晓,陈伟勇
0
(广东海洋大学水产学院,广东 湛江 524088;广东海洋大学水产学院,广东 湛江 524089;广东海洋大学水产学院,广东 湛江 524090;广东海洋大学水产学院,广东 湛江 524091;广东海洋大学水产学院,广东 湛江 524092;广东海洋大学水产学院,广东 湛江 524093;广东海洋大学水产学院,广东 湛江 524094)
摘要:
采用基于高通量测序平台的SLAF seq技术,开发出47个高多态性的黄鳍棘鲷(Acanthopagrus latus)微卫星标记,其中二碱基重复位点17个,三至六碱基重复位点30个。各位点的等位基因数为2~27(均值为10),观测杂合度(Ho)和期望杂合度(He)分别为0.156~0.938(均值为0.682)和0.177~0.963(均值为0.741),多态信息含量(PIC)为0.166~0.946(均值为0.705)。经Bonferroni校正后,有43个位点符合哈迪-温伯格平衡(HWE),其余4个位点偏离HWE。这些多态性微卫星标记为黄鳍棘鲷遗传资源研究提供新的有效分子标记。跨物种扩增结果显示,共有31个黄鳍棘鲷微卫星标记可在9种鲷科鱼类中成功扩增。其中17个标记在太平洋棘鲷(Acanthopagrus pacificus)、黑棘鲷(Acanthopagrus schlegelii)和澳洲棘鲷(Acanthopagrus australis)中具有较好的通用性,这些标记可为棘鲷属(Acanthopagrus)的系统进化和种群遗传学分析提供新的标记来源;另有3个标记在二长棘梨齿鲷(Evynnis cardinalis)、真赤鲷(Pagrus major)、蓝点赤鲷(Pagrus caeruleostictus)及黄牙鲷(Dentex hypselosomus)中具有通用性。
关键词:  海洋生物学  黄鳍棘鲷  微卫星标记  SLAF-seq技术  跨物种扩增
DOI:
基金项目:公益性行业(农业)科研专项资助项目(201403008);广东省科技计划资助项目(2017A030303077);广东省海洋和渔业发展专项(科技攻关与研发)资助项目(A201708D07); 国家自然科学基金资助项目(31372532)
Microsatellite marker development for Acanthopagrus latus and cross species amplification in the family Sparidae
WU Ren-xie,ZHAI Yun,XIAO Yao,NIU Su-fang,ZHANG Hao-ran,LI Xiao,CHEN Wei-yong
(College of Fisheries, Guangdong Ocean University, Zhanjiang 524088, China)
Abstract:
Using the specific-locus amplified fragment sequencing (SLAF-seq) technology, we developed 47 high polymorphic microsatellite markers for Acanthopagrus latus, among which 17 were di-nucleotide repeats and the other 30 were tri- to hexa-nucleotide repeats. The allele number per locus ranged from 2 to 27 (mean 10), and the observed and expected heterozygosities varied from 0.156 to 0.938 (mean 0.682) and 0.177 to 0.963 (mean 0.741), respectively. The polymorphism information contents (PIC) ranged from 0.166 to 0.946 (mean 0.705). After Bonferroni correction, 43 microsatellite loci were consistent with Hardy-Weinberg equilibrium (HWE), while the other 4 loci deviated from the HWE. These polymorphic microsatellite markers could provide new and effective molecular markers for the genetic resources study of A. latus. The results of cross species amplification showed that a total of 31 microsatellite markers could be successfully amplified in 9 species of family Sparidae. Among them 17 were transferable in Acanthopagrus pacificus, Acanthopagrus schlegelii and Acanthopagrus australis, and they would provide new markers for the future studies of phylogenetic relationship and population genetics of these species. The other 3 markers were also transferable in Evynnis cardinalis, Pagrus major, Pagrus caeruleostictus and Dentex hypselosomus.
Key words:  marine biology  Acanthopagrus latus  microsatellite marker  SLAF-seq technology  cross species amplification

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